Journal: Redox Biology
Article Title: MAOB promotes ROS-mediated DNA damage, triggering a cyclic MAOB-HNF1A-53BP1-p53 axis that suppresses the malignancy of clear cell renal cell carcinoma
doi: 10.1016/j.redox.2025.103945
Figure Lengend Snippet: The enzymatic activity of MAOB is pivotal in suppressing growth of ccRCC cells via inducing ROS production. A MAOB protein levels in renal proximal tubule epithelial cells (HK2) and ccRCC cell lines were estimated by Western blotting, with GAPDH as an internal loading control. B MAOB was overexpressed in Caki-1 cells and knocked down in 786-O and HK2 cells, as determined by Western blotting. C–F MAOB expression was negatively correlated with ccRCC cell viability (Caki-1, n = 6; 786-O, n = 6; HK2, n = 4) ( C ), proliferation ( n = 3) ( D ), colony formation (Caki-1, n = 7; 786-O, n = 7; HK2, n = 4) ( E ), and sphere formation ( F ). Data in (C–E) are presented as the mean ± standard deviation (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G Viability of Caki-1/Neo and Caki-1/MAOB cells after treatment with or without 10 μM of various MAOB inhibitors (selegiline, pargyline, or rasagiline) for 48 h ( n = 4). H Viability of Caki-1 cells after transduction with wild-type MAOB, MAOB/Y435W, or a control vector ( n = 6). I, J Oxidative stress were measured using a DCFDA fluorescent probe and flow cytometry in Caki-1/Neo and Caki-1/MAOB cells treated with or without 10 μM selegiline ( n = 3) ( I ) or 5 mM of the antioxidant reagents NAC and GSH ( n = 3) ( J ). K Intracellular H 2 O 2 levels were quantified using the PO-1 fluorescent probe followed by flow cytometric analysis in Caki-1/EV and Caki-1/MAOB cells, with or without treatment with 10 μM selegiline or 5 mM NAC ( n = 3). Exogenous treatment of 200 μM H 2 O 2 was used as a positive control. L Colony-forming ability was evaluated in Caki-1/Neo and Caki-1/MAOB cells treated with or without NAC and GSH. ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared to the vector control group ( n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the MAOB-overexpressing group. For comparisons between two groups, a t -test was performed. For comparisons involving more than two groups, ANOVA followed by Tukey's post hoc test was used.
Article Snippet: The human 786-O, A498, and Caki-1 ccRCC cell lines, as well as kidney tubular epithelial HK2 cells, were sourced from American Type Culture Collection (Manassas, VA, USA).
Techniques: Activity Assay, Western Blot, Control, Expressing, Standard Deviation, Transduction, Plasmid Preparation, Flow Cytometry, Positive Control
ATCC is a verified supplier 
ATCC manufactures this product 
